Spectrophotometry:Article Title: Detection of Cell Death in Planarians
Article Snippet: .. Petri dish (VWR, catalog number: 391-0439) Slides (VWR, catalog number: 631-1551) Razor blade (MARTOR, catalog number: NO. 743) Eppendorf tubes (VWR, Eppendorf, catalog number: 700-5239) 15 ml Falcons (LF Equipamentos, catalog number: 166) Spectrophotometry Cuvettes (VWR, catalog number: 634-0677BTU) 96-well plate (VWR, Corning, catalog number: 734-1664) MilliQ water Ice Micro BCA Protein Assay Kit (Thermo Fisher Scientific, Pierce TM , catalog number: 23235) Tris-HCl, pH 8 (Sigma-Aldrich, catalog number: 93362) EDTA, pH 8 (Sigma-Aldrich, catalog number: 1233508) Triton X-100 (Sigma-Aldrich, catalog number: X100) HEPES pH 7.5 (Sigma-Aldrich, catalog number: H3375) Glycerol 10% (Sigma-Aldrich, catalog number: G5516) DTT (Sigma-Aldrich, catalog number: 646563) Caspase-3 inhibitor Z-DEVD-FMK (Merck, catalog number: 264155) Caspase-3 substrate Ac-DEVD-AMC (BD Biosciences, Pharmingen TM , catalog number: 556449) Lysis buffer (see Recipes) Assay buffer (see Recipes) ..
Bicinchoninic Acid Protein Assay:Article Title: Detection of Cell Death in Planarians
Article Snippet: .. Petri dish (VWR, catalog number: 391-0439) Slides (VWR, catalog number: 631-1551) Razor blade (MARTOR, catalog number: NO. 743) Eppendorf tubes (VWR, Eppendorf, catalog number: 700-5239) 15 ml Falcons (LF Equipamentos, catalog number: 166) Spectrophotometry Cuvettes (VWR, catalog number: 634-0677BTU) 96-well plate (VWR, Corning, catalog number: 734-1664) MilliQ water Ice Micro BCA Protein Assay Kit (Thermo Fisher Scientific, Pierce TM , catalog number: 23235) Tris-HCl, pH 8 (Sigma-Aldrich, catalog number: 93362) EDTA, pH 8 (Sigma-Aldrich, catalog number: 1233508) Triton X-100 (Sigma-Aldrich, catalog number: X100) HEPES pH 7.5 (Sigma-Aldrich, catalog number: H3375) Glycerol 10% (Sigma-Aldrich, catalog number: G5516) DTT (Sigma-Aldrich, catalog number: 646563) Caspase-3 inhibitor Z-DEVD-FMK (Merck, catalog number: 264155) Caspase-3 substrate Ac-DEVD-AMC (BD Biosciences, Pharmingen TM , catalog number: 556449) Lysis buffer (see Recipes) Assay buffer (see Recipes) ..
Lysis:Article Title: Detection of Cell Death in Planarians
Article Snippet: .. Petri dish (VWR, catalog number: 391-0439) Slides (VWR, catalog number: 631-1551) Razor blade (MARTOR, catalog number: NO. 743) Eppendorf tubes (VWR, Eppendorf, catalog number: 700-5239) 15 ml Falcons (LF Equipamentos, catalog number: 166) Spectrophotometry Cuvettes (VWR, catalog number: 634-0677BTU) 96-well plate (VWR, Corning, catalog number: 734-1664) MilliQ water Ice Micro BCA Protein Assay Kit (Thermo Fisher Scientific, Pierce TM , catalog number: 23235) Tris-HCl, pH 8 (Sigma-Aldrich, catalog number: 93362) EDTA, pH 8 (Sigma-Aldrich, catalog number: 1233508) Triton X-100 (Sigma-Aldrich, catalog number: X100) HEPES pH 7.5 (Sigma-Aldrich, catalog number: H3375) Glycerol 10% (Sigma-Aldrich, catalog number: G5516) DTT (Sigma-Aldrich, catalog number: 646563) Caspase-3 inhibitor Z-DEVD-FMK (Merck, catalog number: 264155) Caspase-3 substrate Ac-DEVD-AMC (BD Biosciences, Pharmingen TM , catalog number: 556449) Lysis buffer (see Recipes) Assay buffer (see Recipes) ..
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